MC

Current Trends in Science and Technology

an Open Access Publication ISSN: 0976-9730 | 0976-9498

Chemistry

Covalently Immobilized BSA Protein onto Modified Silicon and Diamond like Carbon Film Surfaces and Optimization for Biosensing

Hari Shankar Biswas, Nihar Ranjan Ray
1Assistant Professor, Department of Chemistry, Surendranath College, 24/2 M.G. Road, Kolkata-700009 2Research Adviser, Department of Chemistry, Surendranath College, 24/2, M.G. Road, Kolkata-700009, Visiting Professor, Centre for Indological Studies and ResearchThe Ramakrishna Mission Institute of Culture, Kolkata-700029
Online First: October 28, 2017
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Abstract

Diamond like carbon (DLC) has an atomically-smooth surface that can be deposited on high-surface area substrata and functionalized with reactive chemical groups, providing an ideal substrate for protein immobilization. A synthetic sequence is described involving deposition and hydrogenation of DLC followed by chemical functionalization.  These functional groups are reacted with amines on proteins causing covalent immobilization on contact. Covalent functionalization of silicon surfaces by electrografting method and subsequent immobilization of bovine serum albumin (BSA). Our intent was to immobilize proteins on the surface of DLC substrate in a site-directed manner while retaining structure and native biological function and optimization for biosensing.  Raman measurements confirm the presence of these surface functional groups and FTIR confirms covalent protein immobilization. Immobilization of BSA onto Silicon surfaces was also verified using FTIR of silicon Silicon surfaces, solution-phase fluorescence. Atomic force microscopy (AFM) of immobilized proteins is reproducible because proteins do not move as a result of interactions with the AFM probe-tip, thus providing an advantage over Silicon substrata typically used in AFM studies of protein. 

Keyword : Silicon, Carbon, Fluorescence, AFM, Diamond, FTIR, Protein

  Submitted
Oct 28, 2017
Published
Oct 28, 2017
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