MC

Current Trends in Science and Technology

an Open Access Publication ISSN: 0976-9730 | 0976-9498

Biotechnology

Isolation and Identification of Sorbitol and Non-Sorbitol Fermenting E. coli Isolated from Vegetable Sources in Keffi Main Market Nasarawa State, Nigeria

Nggada, N.J
Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi
Gberikon, G.M.,
Department of Microbiology, College of Science, University of Agriculture Makurdi, Benue state
Nkene. I.H,
Department of Microbiology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi
Agada, E.O
Department of Microbiology, College of Science, University of Agriculture Makurdi, Benue state
Online First: October 20, 2018
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Abstract

Isolation and identification of sorbitol and non-sorbitol fermenting E. coli isolated from vegetable sources in Keffi main market, Nasarawa State Nigeria was carried out to ascertain level of contamination from these vegetables. A total of hundred (100) samples were collected, twenty (20) each from pumpkin leaves (Telfaria occidentalis), lettuce (Lactuca sativa), spinach (Amaranthus hybridus), cabbage (Brassica oleracea) and carrot (Daucus carota)) were all packaged separately in sterile polythene bags and were immediately transported to Nasarawa state University Microbiology Laboratory for analyses. Sorbitol fermenting E.coli (SFE) and non-sorbitol fermenting E.coli (NSFE) were isolated and identified from the samples using some standard microbiological and biochemical methods. Percentage occurrence of E. coli from one hundred (100) vegetable samples obtained was 58 (58.0%). Percentage occurrence of SFE and NSFE were (41.0%) and NSFE (17.0%) respectively. The percentage occurrence of SFE from vegetable samples were; carrots (60.0%), spinach (45.0%), cabbage (40.0%). Pumpkin and lettuce leaves were (30.0%). For NSFE the percentage occurrence were; lettuce (30.0%), pumpkin and cabbage leaves were (20.0%), carrot (10.0%) and spinach (5.0%) respectively. Health implications of eating unwashed raw vegetables should be emphasized to reduce health related issues.

  Submitted
Oct 20, 2018
Published
Oct 20, 2018
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  • Nggada, N.J
  • Gberikon, G.M.,
  • Nkene. I.H,
  • Agada, E.O

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