Water is an essential resource for all life on the planet. Groundwater is one of the most useful water sources. Contamination of such water sources is a big problem creating health hazards. The water and environment has become an emotive issue with the people and policy makers. The chief causes for the pollution of water and environment are anthropogenic activities of human beings. Hence the present study was undertaken to characterize nature (parameters) of groundwater in Seoni, by taking water samples from eight different stations locating to the Seoni area. Analysis of physico-chemical and microbiological parameters of water samples was carried out during May2017 â€“ April 2018. To assess the quality of groundwater, each parameter was compared with the standard desirable limit of that parameter in drinking water as prescribed by BIS 10500-91. The comparison of different parameters spatially showed an increasing pattern of alkalinity, total dissolved solids, total hardness, calcium, fluoride and fecal coliform concentrations and decreasing concentration of dissolved oxygen in the groundwater.