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Current Trends in Science and Technology

an Open Access Publication ISSN: 0976-9730 | 0976-9498

Biotechnology

Optimizing Tissue Culture Media for Efficient Callus Induction and Regeneration from Rice Seeds

Emica Mary Gatphoh
Centre for Biotechnology, ICAR Research Complex for NEH Region, Umiam-793103.
Arunava Pattanayak
Centre for Biotechnology, ICAR Research Complex for NEH Region, Umiam-793103.
Banshanlang Iangrai
Division of Plant Breeding, ICAR Research Complex for NEH Region, Umiam-793103.
D. Evanoreen Ann Khongwir
Centre for Biotechnology, ICAR Research Complex for NEH Region, Umiam-793103.
Gratify Pale
Centre for Biotechnology, ICAR Research Complex for NEH Region, Umiam-793103.
Mohan Chandra Kalita
Department of Biotechnology, Guwahati University, Guwahati-781014.
Online First: April 24, 2018
| Google Scholar

Abstract

An efficient system was established for high frequency embryogenesis and regeneration of a local variety, NEH Megha Rice1 (Oryza sativa L.).  In the present study Callus was induced on MS (Murashige and Skoog) media supplemented with various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid), 0.5 g/L L-proline, 0.3 g/L casein hydrolysate 30 g/L sucrose and 8 g/L agar. NEH Megha Rice1 showed maximum callus induction frequency on MS medium supplemented with 2.5 mg/L 2,4-D. The regeneration efficiency from healthy embryogenic calli was investigated on MS medium supplemented with various concentrations of BAP (6-Benzylaminopurine) with a fixed concentration of NAA (Naphthalene acetic acid). The regeneration efficiency of calli was also investigated on three different agar concentrations. NEH Megha Rice1 showed maximum regeneration frequency on MS medium supplemented with 5mg/L BAP and 0.05mg/L NAA in 1.2 % agar.

Keyword : NEH Megha Rice1 (Oryza sativa L.), Callus Induction, Plant Regeneration

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Apr 24, 2018
Published
Apr 24, 2018
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References

1. Ahmad, M., Nangyal, H., Imran, M. and Ullah, F. (2016). Optimization of Protocol for Surface Sterilization and Callus Induction for Three Rice Varieties. American-Eurasian J Agric Environ Sci. 16(2): 357-361. 2. Christou, P., Ford, T.L. and Kofron, M. (1991). Production of Transgenic Rice (Oryza sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos. Biotechnol. 9: 957-962. 3. Enriquez-Obregon, G.A., Prieto-Samsonov, D.L., de la Riva, G.A., Perez, M. , Selman-Housein, G. and Vazquez-Padron, R.I. (1999). Agrobacterium-mediated Japonica Rice Transformation: A Procedure Assisted by an Antinecrotic Treatment. Plant Cell Tiss Organ Cult. 59: 159-168. 4. FAO (2006). Food Consumption: Pattern of main food items-share in total dietary energy consumption. FAO (Food and Agricultural Organization of the United States) Statistic Division. Bulletin. 5. Ge, X., Chu, Z., Lin, Y. and Wang, S. (2006). A tissue culture system for different germplasms of indica rice. Plant Cell Rep. 25: 392-402. 6. Ghobeishavi, H., Uliaie, E.D., Alavikia, S.S. and Valizadeh, M. (2015). Study of factors influencing somatic embryogenesis in rice (Oryza sativa L.). Int J Adv Biol Biomed Res. 3(1): 43-50. 7. Goff, S.A., Ricke, D., Lan, T.H., Presting, G., Wang, R., Dunn, M., Glazebrook, J., Sessions, A., Oeller, P., Varma, H. et al. (2002). A Draft Sequence of the Rice Genome (Oryza sativa L. ssp japonica). Sci, 296: 92-100. 8. Gonalz, M.C. (2000). Effects of different growth regulators on in vitro culture of rice cultivors. Tropicales. 21(1): 27-28. 9. Haq, I., Xing, Z.C., Mukhtar, Z., Jaleel, C.A. and Azooz, M.M. (2009). Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati. J Plant Physiol. 166: 1568-1575. 10. Helariutta, Y., Fukaki, H., Wysocka, D.J., Nakajima, K., Jung, J. and Sena, G. (2000 ). The short-root gene controls radial patterning of the Arabidopsis root through radial signaling. Cell. 101(5): 555–567. 11. Hussain, Z., Khan, M.H., Bano, R., Rashid, H. and Chaudhry, Z. (2010). Protocol optimization for efficient callus induction and regeneration in three Pakistani rice cultivars. Pak J Bot. 42(2): 879-887. 12. Kamal, N., Nasuruddin, K.M., Haque, M.S. and Yasmin, S. (2007). Optimization of regeneration protocol of rice from embryo derived callus. Progress. Agric. 18(2): 25-33. 13. Khaleda, L. and Al-Forkan, M. (2006). Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deep water rice (Oryza sativa L.) cultivars of Bangladesh. African J. Biotechnol. 5(16): 1435-1440. 14. Khanna, H.K. and Raina, S.K. (1998). Genotype x culture media interaction effects on regeneration response of three indica rice cultivars. Plant Cell Tiss Organ Cult. 52: 145-153. 15. Kumar, S.S. and Ajinder, K. (2013). Genotype independent tissue culture base line for high regeneration of japonica and indica rice. Res J Biotechnol. 8(12): 96-101. 16. Larkin, P.J. and Scowcraft, W.R. (1981). Somaclonal variation a novel source of variability from cell cultures of plant improvement. Theor Appl Genet. 60: 197-214. 17. Lee, K.S., Jeon, H.S. and Kim, M.Y. (2002). Optimization of a mature embryo based in vitro culture system for high-frequency somatic embryogenic callus induction and plant regeneration from japonica rice cultivars. Plant Cell Tiss Org Cult. 71: 9–13. 18. Lin, Y.Z. and Zhang, Q. (2005). Optimizing the tissue culture conditions for high efficiency transformation of indica rice. Plant Cell Rep. 23: 540-547. 19. Mandal, A.B., Maiti, A. and Biswas, A. (2003). Somatic embryogenesis in root derived callus of indica rice. Plant Tiss Cult. 13: 125-133. 20. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum. 15: 473-497. 21. Navraj, K., Gill, M.S., Raman, G., Bharaj, T.S., Gosal, S.S., Kaur, N. and Gill, R. (1999). Factors enhancing somatic embryogenesis and high frequency plant regeneration in rice. Crop Improvement. 26(1): 23-27. 22. Ozawa, K., Kawahigashi, H., Kayano, T. and Ohkawa, Y. (2003). Enhancement of regeneration of rice (Oryza sativa L.) calli by integration of the gene involved in regeneration ability of the callus. Plant Sci. 165: 395-402. 23. Poraha, R., Poeaim, A., Pongjaroenkit, S. and Pongthongkam, P. (2016). Callus induction and plant regeneration on optimization of the culture conditions in Jow Haw rice (Oryza sativa L.). J Agric Technol. 12(2): 241-248. 24. Ramesh, M. and Gupta, A.K. (2005). Transient expression of β-glucoronidase gene in indica and japonica rice (Oryza sativa L.) callus cultures after different stages of co-bombardment. Afr J Biotechnol. 4(7): 596–600. 25. Rashid, H., Toriyama, K., Quraishi, A., Hinata, K. and Malik, K.A. (2000). An improved method for shoot regeneration from calli of Indica rice. Pak J Biol Sci. 3(12): 2229-2231. 26. Rueb, S., Leneman, M., Schilperoot, R.A., Lan, H. (1994). Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryo. Plant cell Tiss Organ Cult. 36: 259-264. 27. Saharan, V., Yadav, R.C., Yadav, N.R. and Chapagain, B.P. (2004). High frequency plant regeneration from desiccated calli of indica rice (Oryza Sativa l.). African J Biotechnol. 3(5): 256-259. 28. Sahrawat, A.K. and Chand, S. (2001). High-frequency plant regeneration from coleoptile tissue of indica rice (Oryza sativa L.). In Vitro Cell Dev Biol Plant. 37: 55-61. 29. Sankepally, S.S.R. and Singh, B. (2016). Optimization of regeneration using differential growth regulators in indica rice cultivars. 3 Biotech. 6(19): 1-7. 30. Toppo, E., Ramakrisgnan, M., Ceasar, A.S., Sivasankaran, K., Premkumar, A. and Ignacimuthu, S. (2014). Regeneration from mature scutellum explants of rice variety IR64 (Oryza sativa L.) through direct and indirect organogenesis. J Global Agric Ecol. 1(1): 1-9. 31. Torbert, K.A., Rines, H.W. and Somers, D.A. (1998). Transformation of oat using mature embryo- derived tissue cultures. Crop Sci. 38: 226-231. 32. Tyagi, H., Rajasubramaniam, S. and Dasgupta, I. (2007). Regeneration and Agrobacterium-mediated transformation of a popular indica rice variety ADT39. Curr Sci. 93: 678-683. 33. Valdez, M., Monoz, M., Vega, J.R. and Espinoza, A.M. (1997). Plant regeneration of Indica rice from mature embryo derived calli. Revista De Biologia Tropical. 44(3): 13-21. 34. Verma, D., Joshi, R., Shukla, A. and Kumar, P. (2011). Protocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.). Ind J Exp Biol. 49: 958–963. 35. Visarada, K.B.R., Sailaja, S.M. and Sarma, N.P. (2002). Effect of callus induction media on morphology of embryogenic calli in rice genotypes. Biolgia Plantarum. 45: 495-502. 36. Xie, J.H., Gao, M.W., Cai, Q.H., Chens, X.Y., Shen, Y.W. and Liang, Z.Q. (1995). Optimized growth regulators combination in Japonica rice. Plant Cell Tiss Organ Cult. 42(3): 245-250. 37. Yamada, Y., Yang, T.Q. and Tang, D.T. (1986). Plant regeneration from protoplast derived callus of rice (Oryza sativa L.). Plant Cell Rep. 4: 85-88. 38. Yu, J., Hu, S., Wang, J., Wong, G.K., Li, S., Liu, B., Deng, Y., Dai, L., Zhou, Y., Zhang, X. and others. (2002). A Draft Sequence of the Rice Genome (Oryza sativa L. ssp. indica). Sci. 296: 79-92.
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References

1. Ahmad, M., Nangyal, H., Imran, M. and Ullah, F. (2016). Optimization of Protocol for Surface Sterilization and Callus Induction for Three Rice Varieties. American-Eurasian J Agric Environ Sci. 16(2): 357-361.
2. Christou, P., Ford, T.L. and Kofron, M. (1991). Production of Transgenic Rice (Oryza sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos. Biotechnol. 9: 957-962.
3. Enriquez-Obregon, G.A., Prieto-Samsonov, D.L., de la Riva, G.A., Perez, M. , Selman-Housein, G. and Vazquez-Padron, R.I. (1999). Agrobacterium-mediated Japonica Rice Transformation: A Procedure Assisted by an Antinecrotic Treatment. Plant Cell Tiss Organ Cult. 59: 159-168.
4. FAO (2006). Food Consumption: Pattern of main food items-share in total dietary energy consumption. FAO (Food and Agricultural Organization of the United States) Statistic Division. Bulletin.
5. Ge, X., Chu, Z., Lin, Y. and Wang, S. (2006). A tissue culture system for different germplasms of indica rice. Plant Cell Rep. 25: 392-402.
6. Ghobeishavi, H., Uliaie, E.D., Alavikia, S.S. and Valizadeh, M. (2015). Study of factors influencing somatic embryogenesis in rice (Oryza sativa L.). Int J Adv Biol Biomed Res. 3(1): 43-50.
7. Goff, S.A., Ricke, D., Lan, T.H., Presting, G., Wang, R., Dunn, M., Glazebrook, J., Sessions, A., Oeller, P., Varma, H. et al. (2002). A Draft Sequence of the Rice Genome (Oryza sativa L. ssp japonica). Sci, 296: 92-100.
8. Gonalz, M.C. (2000). Effects of different growth regulators on in vitro culture of rice cultivors. Tropicales. 21(1): 27-28.
9. Haq, I., Xing, Z.C., Mukhtar, Z., Jaleel, C.A. and Azooz, M.M. (2009). Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati. J Plant Physiol. 166: 1568-1575.
10. Helariutta, Y., Fukaki, H., Wysocka, D.J., Nakajima, K., Jung, J. and Sena, G. (2000 ). The short-root gene controls radial patterning of the Arabidopsis root through radial signaling. Cell. 101(5): 555–567.
11. Hussain, Z., Khan, M.H., Bano, R., Rashid, H. and Chaudhry, Z. (2010). Protocol optimization for efficient callus induction and regeneration in three Pakistani rice cultivars. Pak J Bot. 42(2): 879-887.
12. Kamal, N., Nasuruddin, K.M., Haque, M.S. and Yasmin, S. (2007). Optimization of regeneration protocol of rice from embryo derived callus. Progress. Agric. 18(2): 25-33.
13. Khaleda, L. and Al-Forkan, M. (2006). Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deep water rice (Oryza sativa L.) cultivars of Bangladesh. African J. Biotechnol. 5(16): 1435-1440.
14. Khanna, H.K. and Raina, S.K. (1998). Genotype x culture media interaction effects on regeneration response of three indica rice cultivars. Plant Cell Tiss Organ Cult. 52: 145-153.
15. Kumar, S.S. and Ajinder, K. (2013). Genotype independent tissue culture base line for high regeneration of japonica and indica rice. Res J Biotechnol. 8(12): 96-101.
16. Larkin, P.J. and Scowcraft, W.R. (1981). Somaclonal variation a novel source of variability from cell cultures of plant improvement. Theor Appl Genet. 60: 197-214.
17. Lee, K.S., Jeon, H.S. and Kim, M.Y. (2002). Optimization of a mature embryo based in vitro culture system for high-frequency somatic embryogenic callus induction and plant regeneration from japonica rice cultivars. Plant Cell Tiss Org Cult. 71: 9–13.
18. Lin, Y.Z. and Zhang, Q. (2005). Optimizing the tissue culture conditions for high efficiency transformation of indica rice. Plant Cell Rep. 23: 540-547.
19. Mandal, A.B., Maiti, A. and Biswas, A. (2003). Somatic embryogenesis in root derived callus of indica rice. Plant Tiss Cult. 13: 125-133.
20. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum. 15: 473-497.
21. Navraj, K., Gill, M.S., Raman, G., Bharaj, T.S., Gosal, S.S., Kaur, N. and Gill, R. (1999). Factors enhancing somatic embryogenesis and high frequency plant regeneration in rice. Crop Improvement. 26(1): 23-27.
22. Ozawa, K., Kawahigashi, H., Kayano, T. and Ohkawa, Y. (2003). Enhancement of regeneration of rice (Oryza sativa L.) calli by integration of the gene involved in regeneration ability of the callus. Plant Sci. 165: 395-402.
23. Poraha, R., Poeaim, A., Pongjaroenkit, S. and Pongthongkam, P. (2016). Callus induction and plant regeneration on optimization of the culture conditions in Jow Haw rice (Oryza sativa L.). J Agric Technol. 12(2): 241-248.
24. Ramesh, M. and Gupta, A.K. (2005). Transient expression of β-glucoronidase gene in indica and japonica rice (Oryza sativa L.) callus cultures after different stages of co-bombardment. Afr J Biotechnol. 4(7): 596–600.
25. Rashid, H., Toriyama, K., Quraishi, A., Hinata, K. and Malik, K.A. (2000). An improved method for shoot regeneration from calli of Indica rice. Pak J Biol Sci. 3(12): 2229-2231.
26. Rueb, S., Leneman, M., Schilperoot, R.A., Lan, H. (1994). Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryo. Plant cell Tiss Organ Cult. 36: 259-264.
27. Saharan, V., Yadav, R.C., Yadav, N.R. and Chapagain, B.P. (2004). High frequency plant regeneration from desiccated calli of indica rice (Oryza Sativa l.). African J Biotechnol. 3(5): 256-259.
28. Sahrawat, A.K. and Chand, S. (2001). High-frequency plant regeneration from coleoptile tissue of indica rice (Oryza sativa L.). In Vitro Cell Dev Biol Plant. 37: 55-61.
29. Sankepally, S.S.R. and Singh, B. (2016). Optimization of regeneration using differential growth regulators in indica rice cultivars. 3 Biotech. 6(19): 1-7.
30. Toppo, E., Ramakrisgnan, M., Ceasar, A.S., Sivasankaran, K., Premkumar, A. and Ignacimuthu, S. (2014). Regeneration from mature scutellum explants of rice variety IR64 (Oryza sativa L.) through direct and indirect organogenesis. J Global Agric Ecol. 1(1): 1-9.


31. Torbert, K.A., Rines, H.W. and Somers, D.A. (1998). Transformation of oat using mature embryo- derived tissue cultures. Crop Sci. 38: 226-231.
32. Tyagi, H., Rajasubramaniam, S. and Dasgupta, I. (2007). Regeneration and Agrobacterium-mediated transformation of a popular indica rice variety ADT39. Curr Sci. 93: 678-683.
33. Valdez, M., Monoz, M., Vega, J.R. and Espinoza, A.M. (1997). Plant regeneration of Indica rice from mature embryo derived calli. Revista De Biologia Tropical. 44(3): 13-21.
34. Verma, D., Joshi, R., Shukla, A. and Kumar, P. (2011). Protocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.). Ind J Exp Biol. 49: 958–963.
35. Visarada, K.B.R., Sailaja, S.M. and Sarma, N.P. (2002). Effect of callus induction media on morphology of embryogenic calli in rice genotypes. Biolgia Plantarum. 45: 495-502.
36. Xie, J.H., Gao, M.W., Cai, Q.H., Chens, X.Y., Shen, Y.W. and Liang, Z.Q. (1995). Optimized growth regulators combination in Japonica rice. Plant Cell Tiss Organ Cult. 42(3): 245-250.
37. Yamada, Y., Yang, T.Q. and Tang, D.T. (1986). Plant regeneration from protoplast derived callus of rice (Oryza sativa L.). Plant Cell Rep. 4: 85-88.
38. Yu, J., Hu, S., Wang, J., Wong, G.K., Li, S., Liu, B., Deng, Y., Dai, L., Zhou, Y., Zhang, X. and others. (2002). A Draft Sequence of the Rice Genome (Oryza sativa L. ssp. indica). Sci. 296: 79-92.
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  • Emica Mary Gatphoh
  • Arunava Pattanayak
  • Banshanlang Iangrai
  • D. Evanoreen Ann Khongwir
  • Gratify Pale
  • Mohan Chandra Kalita

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