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Current Trends in Science and Technology

an Open Access Publication ISSN: 0976-9730 | 0976-9498

Pharmaceutical Sciences

Simple and rapid liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for estimation of Ondansetron HCl in dog plasma

Ramakant Kashinath Gundu, Sanjay S. Pekamwar
School of Pharmacy, Swami Ramanand Teerth Marathwada University,Vishnupuri, Nanded, Maharashtra 431606, India E-mail: rkgundu@gmail.com
Online First: March 03, 2018
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Abstract

A simple and rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed and applied for estimation of Ondansetron concentration levels in dog plasma after administration of Ondansetron tablets. The drug and Internal standard, ISTD (Trospium HCl) were extracted from plasma by protein precipitation technique. The analytes were separated using a mobile phase of ammonium formate (10 mM) and organic mixture containing Acetonitrile and Methanol (20:80, v/v) on Zorbax SB C18, 75 x 2.1 mm, 3.5µm with guard column at a flow rate of 0.3 mL/min and analyzed by a triple-quadrupole mass spectrometer in positive ion multiple reaction monitoring (MRM) mode at transitions (m/z) of 294.0 ® 170.1 for Ondansetron and 392.2®182 for ISTD, respectively. The total chromatographic run time was 3.5 min and the assay exhibited a linear dynamic range of 0.5 - 100 ng/mL for Ondansetron in dog plasma. The developed method was successfully employed to support the bioavailability / pharmacokinetic study of Ondansetron formulations in dogs.

Keyword : Ondansetron; LC-MS/MS; dog plasma; pharmacokinetics

  Submitted
Mar 3, 2018
Published
Mar 3, 2018
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References

1. Moreira, R.F., M.C. Salvadori, C.P. Azevedo, D. Oliveira‐Silva and D.C. Borges, Development and validation of a rapid and sensitive LC‐ESI‐MS/MS method for ondansetron quantification in human plasma and its application in comparative bioavailability study. Biom. Chromatography, 2010, 24: 1220-1227. 2. http://www.rxlist.com/ondansetron-hydrochloride-drug.htm 3. Lingxia Pang, Qing Wang, Youpei Wang , Meiqin Zheng , Haiya Wu, Zhiyi Wang. Development and Validation of LC-MS/MS Method forDetermination of Ondansetron in rat Plasma and its Application. 2012, 31 (2), 305-309. 4. Zhou Mei, Sun Gang,Wu Gu. Pharmacokinetics and bioavailability of orally disintegrating tablets of ondansetron hydrochloride in human. Anhui Yiyao, 2012, 16 (3), 314-316 5. Jean-Claude Alvarez, Beny Charbit, Stanislas Grassin-Delyle, Jean-Louis Demolis, Christian Funck-Brentano, Emuri Abe,Human plasma quantification of droperidol and ondansetron used in preventing postoperative nausea and vomiting with a LC/ESI/MS/MS method,Journal of Chromatography B,879(2),2011, 186-190. 6. Moreira, R. F., Salvadori, M. C., Azevedo, C. P., Oliveira-Silva, D., Borges, D. C., Moreno, R. A., Sverdloff, C. E. and Borges, N. C. (2010), Development and validation of a rapid and sensitive LC-ESI-MS/MS method for ondansetron quantification in human plasma and its application in comparative bioavailability study. Biomed. Chromatogr., 24: 1220–1227. 7. Yannis Dotsikas, Constantinos Kousoulos, Georgia Tsatsou, Yannis L. Loukas,Development and validation of a rapid 96-well format based liquid–liquid extraction and liquid chromatography–tandem mass spectrometry analysis method for ondansetron in human plasma,Journal of Chromatography B,Volume 836, Issues 1–2,2006,
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References

1. Moreira, R.F., M.C. Salvadori, C.P. Azevedo, D. Oliveira‐Silva and D.C. Borges, Development and validation of a rapid and sensitive LC‐ESI‐MS/MS method for ondansetron quantification in human plasma and its application in comparative bioavailability study. Biom. Chromatography, 2010, 24: 1220-1227.
2. http://www.rxlist.com/ondansetron-hydrochloride-drug.htm
3. Lingxia Pang, Qing Wang, Youpei Wang , Meiqin Zheng , Haiya Wu, Zhiyi Wang. Development and Validation of LC-MS/MS Method forDetermination of Ondansetron in rat Plasma and its Application. 2012, 31 (2), 305-309.
4. Zhou Mei, Sun Gang,Wu Gu. Pharmacokinetics and bioavailability of orally disintegrating tablets of ondansetron hydrochloride in human. Anhui Yiyao, 2012, 16 (3), 314-316
5. Jean-Claude Alvarez, Beny Charbit, Stanislas Grassin-Delyle, Jean-Louis Demolis, Christian Funck-Brentano, Emuri Abe,Human plasma quantification of droperidol and ondansetron used in preventing postoperative nausea and vomiting with a LC/ESI/MS/MS method,Journal of Chromatography B,879(2),2011, 186-190.
6. Moreira, R. F., Salvadori, M. C., Azevedo, C. P., Oliveira-Silva, D., Borges, D. C., Moreno, R. A., Sverdloff, C. E. and Borges, N. C. (2010), Development and validation of a rapid and sensitive LC-ESI-MS/MS method for ondansetron quantification in human plasma and its application in comparative bioavailability study. Biomed. Chromatogr., 24: 1220–1227.
7. Yannis Dotsikas, Constantinos Kousoulos, Georgia Tsatsou, Yannis L. Loukas,Development and validation of a rapid 96-well format based liquid–liquid extraction and liquid chromatography–tandem mass spectrometry analysis method for ondansetron in human plasma,Journal of Chromatography B,Volume 836, Issues 1–2,2006,
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