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Current Trends in Science and Technology

an Open Access Publication ISSN: 0976-9730 | 0976-9498

Botany (Plant Science)

In Vitro Effect of Various Systematic Fungicides on Inhibition Spore Germination

Khursheed Aalum, Imtiyaz Ahmad Wani, S.D Singh, Sujata Ganguly, Bharti Kumar
PhD Research scholar, department of botany, M.V.M College Bhopal, India1 PhD Research scholar, department of botany, M.V.M College Bhopal, India2 Assistant professor, department of botany, M.V.M College Bhopal, India3 Professor, department of botany, M.V.M College Bhopal, India4 Assistant professor, department of botany, M.V.M College Bhopal, India5
Online First: February 07, 2018
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Abstract

To ascertain the efficacy of different fungicides, the experiments were conducted in a laboratory. Slide germination technique (SGT) was employed to test the fungicides for inhibition of spore germination. Spore suspension of fungus was prepared by adding 10 ml of sterile distilled water to 10 days old well grown fungal slants. The concentration was adjusted to 50 spores per microscopic field at 10 x power of magnification by further dilutions with sterile distilled water. Equal volumes of the given fungicide respectively for each concentration and the spore suspension were thoroughly mixed. One drop of this was placed in sterilized cavity slide. These slides were placed in moist chambers from a pair of petriplates carrying wet blotting paper to maintain adequate humidity and incubated at 25 +_ 10 c for 24 hours. Each treatment was replicated three times for each concentration. In case of control only sterile water was added instead of fungicide. Observations were recorded after 24 hours Germinated and ungerminated spores were counted separately and the per cent spore germination was calculated.

Keyword : Fungicides, Alternaria leaf spot, slide germination technique

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Feb 7, 2018
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Feb 7, 2018
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References

1. Anonymous. “Package of practices for temperate fruits”. Directorate of Extension Education SKUAST-K, Shalimar campus- 191121 (2002). 2. Asari, M. and S. Takahashi. “ Occurrence of iprodione-resistant strains of Alternaria mali Roberts on apple”. Bulletin of the Akita Fruit Tree Experiment Station, Japan 19(1988):13-24. 3. Dickens, J. S. W., and R. T. A. Cook. "Japanese pear black spot and apple alternaria blotch." EPPO Bulletin 25.4 (1995): 651-659. 4. Dickens,J.S.W.; Cook R.T.A . “Alternaria pear black spot and apple blotch”.Bulletin OEPP/EPPO Bulletin 25 (1995) . 5. Filajdic N, Sutton TB.” Identification and distribution of Alternaria mali on apples in North Carolina and susceptibility of different varieties of apple to Alternaria blotch”. Plant Dis. 75(1991):1045-1048. 6. Filajdic.N and Sutton.T.B.”Chemical control of Alternaria blotch of apples caused by Alternaria mali”.Plant Disease 76 (1992):1126-130. 7. Gagkaeva, T. Y., and M. M. Levitin. "Identification of the pathogen causing leaf spots on apple trees in Krasnodar province orchards." Mikologiya i Fitopatologiya 34.3 (2000): 58-62. 8. K. L. Chadha and R. P. Awasthi. “The Apple.” Malhotra Publishing House, New Delhi (2004):511. 9. Kohmoto, K. , Taniguchi, T . , Nishimura, S. “Correlation between the susceptibility of apple cuitivars to Alternaria mali and their sensitivity to AMtoxin 1”. Ann. Phytopathol. Soc. Japan 43(1977):65-66.
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References

1. Anonymous. “Package of practices for temperate fruits”. Directorate of Extension Education SKUAST-K, Shalimar campus- 191121 (2002).
2. Asari, M. and S. Takahashi. “ Occurrence of iprodione-resistant strains of Alternaria mali Roberts on apple”. Bulletin of the Akita Fruit Tree Experiment Station, Japan 19(1988):13-24.
3. Dickens, J. S. W., and R. T. A. Cook. "Japanese pear black spot and apple alternaria blotch." EPPO Bulletin 25.4 (1995): 651-659.
4. Dickens,J.S.W.; Cook R.T.A . “Alternaria pear black spot and apple blotch”.Bulletin OEPP/EPPO Bulletin 25 (1995) .
5. Filajdic N, Sutton TB.” Identification and distribution of Alternaria mali on apples in North Carolina and susceptibility of different varieties of apple to Alternaria blotch”. Plant Dis. 75(1991):1045-1048.
6. Filajdic.N and Sutton.T.B.”Chemical control of Alternaria blotch of apples caused by Alternaria mali”.Plant Disease 76 (1992):1126-130.
7. Gagkaeva, T. Y., and M. M. Levitin. "Identification of the pathogen causing leaf spots on apple trees in Krasnodar province orchards." Mikologiya i Fitopatologiya 34.3 (2000): 58-62.



8. K. L. Chadha and R. P. Awasthi. “The Apple.” Malhotra Publishing House, New Delhi (2004):511.
9. Kohmoto, K. , Taniguchi, T . , Nishimura, S. “Correlation between the susceptibility of apple cuitivars to Alternaria mali and their sensitivity to AMtoxin 1”. Ann. Phytopathol. Soc. Japan 43(1977):65-66.
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