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Current Trends in Science and Technology

an Open Access Publication ISSN: 0976-9730 | 0976-9498

Life Science

Studies on Effect of Nutritional Parameters on Azo-Dye Decolorizing Immobilized yeast cells

Mohammed Akram Ali Ansari, Dastagir Mohammed, P. Naga Padma
Mohammed Akram Ali Ansari1, Dastagir Mohammed1, P. Naga Padma1* 1Department of Microbiology, Bhavan’s Viveknanda College, Secunderabad-94, India. Corresponding Author email: naga_padmathota@yahoo.com
Online First: December 01, 2017
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Abstract

Azo dyes are recalcitrant xenobiotic compounds used extensively in textile industries. Textile industries generate and discharge millions of liters of untreated Azo-dye containing effluents in the form of wastewater, which cannot and is not totally degraded by conventional wastewater treatment processes. This, thus causes  a serious damage to the environment and needs biological treatment. Degradation of azo dyes being a necessity, diverse dye dumped soils were screened for dye degrading microorganisms especially yeasts. Yeast was only screened as they are efficient degraders and also GRAS organisms. One efficient yeast isolate identified as Saccharomyces sp. which is capable of decolorizing both mono and di-azo dyes was selected, immobilized and studied for azo dye decolourization. The entrapped yeast cells were studied for decolourization of commercially available textile dyes having azo groups. The immobilized yeast’s decolourization abilities were different and varied with different concentrations of carbon and nitrogen sources. The decolourization ranged from 70-100%. Complete decolourization was seen within 12 and 18 hours depending on bead size, number and also the nutrient source. The immobilized beads of size 0.02  cu.sq.mm,  numbering 20 for 20ml medium with 106 yeasts cells  could be reused efficiently for three cycles. The dye degrading immobilized Saccharomyces isolate could be commercially significant as it is efficiently degrading both mono and di-azo dyes, dye waste waters in a semi-continuous manner and so can be used for industrial effluent treatment.

Keyword : Azo dyes, Mono-azo dye, Saccharomyces sps, immobilization, dye decolourization.

  Submitted
Dec 24, 2017
Published
Dec 1, 2017
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