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Current Trends in Science and Technology

an Open Access Publication ISSN: 0976-9730 | 0976-9498

Botany (Plant Science)

Isolation, Characterization and Identification of Pgpr Isolates from Rhizosphere of Barley Plants (Hordeum Vulgare) in Burdwan

Medhatithi Sen, Sikha Dutta
1The UGC Centre for Advanced Study, Department of Botany, The University of Burdwan, Burdwan, 713104, West Bengal, India. *Corresponding author Email id: sikha.bu.academia@gmail.com
Online First: December 16, 2017
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Abstract

Plant growth promoting rhizobacteria (PGPR) a large    group of bacteria that have been occurs in the rhizosphere, in association with roots which can increase plant growth   directly or indirectly and crop yield also. In present communication we have isolatd and characterized a PGPR bacterium from the rhizospheric soil of Hordeum vulgare (L.). Among the properties by which PGPR exert beneficial effects on plants are facilitating the uptake of nutrients such as phosphorus via phosphate solubilization, synthesizing stimulatory phytohormones like (IAA) Vessey, J.K. (2003)


The PGPR traits of the said bacterium were analysed.IAA production; production of HCN, ammonia, polysaccharide, siderophore, nitrogen fixation etc and the assay of the enzymes like protease, nitrogenase, catalase etc was done. Bioactivities like antifungal and as well as stress tolerance property like heavy metal tolerance and salt   tolerance was tested.


Molecular identification of the bacterium was done by 16s r-RNA sequencing .Phylogenetic tree was prepared and Phylogenetic analysis of this PGPR bacterium has confirmed that it is Burkholderia   fungorum.

Keyword : : IAA, HCN, phosphate solubilization, 16 s rRNA sequencing

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Dec 16, 2017
Published
Dec 16, 2017
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